Modification of alprazolam-induced liver injury by bone marrow-derived mesenchymal stem cells and the role of miRNA-192

The group of drugs known as Benzodiazepines (BDZs) are among the most widely prescribed CNS-depressant drugs. Alprazolam (Alp) is a member BDZs family, commonly an antipsychotic and anxiolytic agent. Induction oxidative stress, impairment cognitive functions psychomotor skills, conformational alterations in hemoglobin structure elevation liver enzymes side effects reported on use alprazolam. Several studies have found that alprazolam could favor hepatotoxicity, whereas other contradicted those findings. Bone marrow-derived mesenchymal stem cells (BM-MSCs) been studied novel approach for treatment diseases. current study was designed to assess biochemical, histopathological molecular response oral administration at dose 0.3 mg/kg/day 4 weeks adult male albino rats evaluate therapeutic effect BM-MSCs alprazolam- induced alterations. Forty (Sprague Dawley strain; 170-200 g mean body weight) were used. Liver measured, isolation preparation BM- MSCs done, immunohistochemical staining alpha smooth muscle actin FGF2 assessed. Moreover, histological ultrastructural tissue examination PCR detection SOD, TNF-α mirNA-192 investigated. Animals exposed developed injury characterized by significant increase decrease SOD miRNA- 192 expression. Histological findings provided supportive evidence biochemical analyses. Treatment with caused alleviation In conclusion, induce which ameliorated administration.